Phytochemical profiling using LC-Q-TOF-MS analysis and in vitro antioxidant activtiy of a rere salt-secreting mangrove aegialitis rotundifolia roxb leaves extract
By: Ghosh, Debjit.
Contributor(s): Mondal, Sumanta | Ramakrishna, K.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol. 2(3).Description: 37-47p.Subject(s): PHARMACEUTICSOnline resources: Click here In: International journal of pharmacy and pharmaceutical scienceSummary: Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro . Metho ds: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole -time -of -flight mass spectrometry (LC -Q-TOF -MS ). The antioxidant properties were investigated co mprehensively using seven in vitro models viz., 2, 2 -diphenyl -1-picrylhydrazyl ( DPPH ) radical scavenging, n itric oxide (NO) scavenging, hydrogen peroxide (H 2 O 2 Result s: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC -Q-TOF -MS analysis detected 2 5 phytoconstituents in the extract mostly belonging to flavon oids and alkaloids . The test extract showed strong dose -dependent antioxidant activity in all the seven in vitro models, however , the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. ) scavenging, superoxide (SOD) radical scavenging, l ipid peroxidation (LPO) assay, reducing power (RP), and t otal antioxidant activity. Conclusio n: The test extrac t showed strong antioxidant properties which could be possibly due to the phytoconst ituents detected in the extract.Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|
Articles Abstract Database | School of Pharmacy Archieval Section | Not for loan | 2018558 |
Objective:
The present work deals with the qualitative study of the phytoconstituents
present in
Aegialitis rotundifolia
Roxb., ethanolic leaves
extract
and
evaluate its antioxidant properties
in vitro
.
Metho
ds:
The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by
liquid
chromatography quadrupole
-time
-of -flight mass spectrometry
(LC
-Q-TOF
-MS
). The antioxidant properties
were
investigated co
mprehensively
using seven
in vitro
models viz., 2, 2
-diphenyl
-1-picrylhydrazyl (
DPPH
) radical scavenging, n
itric oxide (NO) scavenging, hydrogen peroxide (H
2
O
2
Result
s:
The preliminary phytochemical analysis revealed the presence of several
important
phytochemical groups whereas the LC
-Q-TOF
-MS
analysis detected 2
5 phytoconstituents
in the extract
mostly belonging
to flavon
oids and alkaloids
. The test extract showed strong
dose
-dependent
antioxidant activity in all the seven
in vitro
models,
however
, the activity of the extracts was
slightly lower compared to the reference standard
ascorbic acid.
)
scavenging,
superoxide (SOD) radical scavenging, l
ipid peroxidation (LPO) assay,
reducing power
(RP), and t
otal antioxidant activity.
Conclusio
n:
The
test extrac
t showed
strong antioxidant properties which
could be possibly due to the phytoconst
ituents
detected in the extract.
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